Stop the current collection in SerialEM.

Save the Navigator and Log file of the current run.

Close SerialEM.

Put the Grating grid onto the stage using the Cryospectporter window and the command “Load”.

Remove all Cartridges from the Sample Storage.

Switch off Emission by hovering over “Emission” in the TEM center microscope pictogram and click in the line Auto Emission “OFF”

Wait until emission reached 0 uA.

Switch off High Tension by clicking “HT” in the TEM center microscope pictogram and click in the line for HT on “OFF”.

Wait until you reached 0 kv high tension

In the TEM center open the window “LN2 Auto Refill Maintenance”.

Click on “Heater Controller”.

In the new window select the finish date and time (normally Monday morning, 7 am).

Ensure that the Cooling time is set to 8 hours.

Ensure that the all targets are selected (i.e. Column, Sample Storage and Stage).

Click in the section “Bake Out operation” on “Start”.

In the pop_up window confirm with “Yes”.

The bakeout will last until the selected time

In Digital Micrograph select in the menu bar “Camera –> Temperature” (Power User mode must be enabled).

In the confirmation window select “Yes”.

In the next window select a target temperature of 50 degrees celsius. Click “OK”.

In the next window click on “Options…”.

Select a Heating duration of 8 hours and a Start delay of 1 hour.

Confirm with “Ok”.

Click on “Start” to run the heating cycle of the camera.