BECM is organizing the second edition of its Cryo-EM workshop. This course is mainly targeted at novice users and people that want to get an introduction into Cryo-EM its possibilities and its limitations. The course is spread over three days and covers the basics of the technology from image formation, sample preparation, imaging and data processing. During the first two days of the workshop practical hands-on demos and lectures will be given. In the practical parts you will be able to handle the cryo-plunger, the screening microscopes (JEOL1400) and the high-end microscope (CryoARM 300). In addition you will get a hand-on introduction to data processing. On the third day you will join the user meeting in which you will be introduced to real life projects and solutions during lectures and poster presentations.

Location:

Building I, Room 0.03

VUB Campus Brussels

Pleinlaan 2

1050 Brussels

Schedule (to be finalized): 

Wednesday 14th September

9:00 - 13:00    Introductory lectures to image formation and sample preparation (w. coffee breaks)

13:00 - 14:00  Lunch break

14:00 - 17:00  Practical demos and hands-on, location: BECM building P1T

                       Part 1: Grid preparation: cryo plunging (Gatan CP-3) and negative staining

                       Part 2: Screening and usage of a mid tier transmission electron microscope: Using the JEOL 1400 and imaging of the cryo grids

                       Part 3: How to run a high-end data collection: Data collection on the JEOL CryoARM 300

Thursday 15th September

9:00 - 13:00    Introductory lectures to imaging and data processing (w. coffee breaks)

13:00 - 14:00  Lunch break

14:00 - 17:00  Practical demos and hands-on: location: Computer room E1.05

                       Data processing with access to individual (or max 2 p. shared) processing station using CryoSparc.

Friday 16th September (w. user meeting)

9:00 - 9:45       Keynote: Yaser Hashem (Institut de Européen de Chimie et Biologie (IECB), Bordeaux)

9:45 - 10:30     JEOL speaker (remote presentation)

10:30 - 11:00   coffee break

11:00 - 11:20   Janine Brunner (VUB)

11:20 - 11:40   Kenneth Verstraete (UGhent)

11:40 - 12:00   Quinten Goovaerts (Leuven)

12:00 - 12:20   Han Remaut lab (VUB)

12:20 - 12:40   Saskia Lippens (VIB)  

12:40 - 14:00   Lunch break w. posters

14:00 - 14:20   Petya Krastova (Bordeaux)  

14:20 - 14:40   Gangadhar Eluru (VUB)

14:40 - 15:00   Last words

15:00 - 16:00   Posters and drinks

16:00 -             Dinner and drinks (Appetizers and burgers cooked on site)

To register for the workshop please fill out this form: 

Registration link (Please note that the workshop is filled to capacity. Applications will only be accepted in case of short term dropouts)

Registration for the workshop is on a first-come basis and participant numbers are limited due to the practical hands-on demonstrations.

Registration fees:

65 EUR for academics (this includes a 1-year membership to the Belgian Biophysical Society)

50 EUR for academic members of the Belgian Biophysical Society

250 EUR for industry

Please send the registration fees with you name and BECM workshop in the reference to:

Marcus Fislage

IBAN: BE04 3631 7195 1031

BIC: BBRUBEBB

For questions please contact mfislage@vub.be